Download Cumulative Subject Index to Volumes 61-74, 76-80 by Martha G. Dennis, Edward A. Dennis PDF

By Martha G. Dennis, Edward A. Dennis

Quantity ninety five presents a handy index to the contents of Volumes sixty one via seventy four and Volumes seventy six via eighty. The nomenclature and utilization within the unique articles were retained at any place attainable, aiding the reader in finding details. ''Notes at the Use of the Index'' presents a worthy dialogue of the incidence of entries for a given topic below a number of names and phraseologies and the order of entries within the index

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P n e u m o n i a e , 69, 50, 51 transport, 69, 720-721 Ammonium pentachlorooxomolybdate reagent, 72, 189 Ammonium persulfate, 65, 121, 241, 302, 303, 309, 452, 540-543, 573, 671, 685, 782, 800, 809 cytochrome assay, 69, 182 Ammonium 1-pyrrolidine dithiocarbamate, Mn extraction, 69, 362 Ammonium sulfate analysis, of 32p-labeled RNA fragments, 65, 673, 674, 676 fractionation, preparation of immunoglobulin G, 74, 26, 67, 267, 285, 413,440 high molecular weight hemoglobin precipitation by, 76, 51-53 inhibition, 63, 37 purification of CAP, 65, 859, 862 of E.

C r a s s a endo-exonuclease, 65, 262 ionization effect on metal binding, 63, 259-261 metal-ion complex, 63, 267-269, 305-318 as nonheme ligand, 76, 471 binding studies, 76, 542-544, 557 effect on oxygen equilibria, 76, 520 oxygen-18 labeled, 64, 67 protons, 69, 712 reaction mixture, for cell-free protein synthesis, 65, 806 of DNA ligase, 65, 883 of T4 RNA ligase, 65, 68, 71 regeneration system, 64, 320 reverse electron transport luminescence, 69, 637, 638 oxidation of cytochrome f, 69, 634 properties, 69, 639 reduction of Q, 69, 632-634 simultaneous measurement of ApH and Q reduction, 69, 634-636 substrate inhibition, 63, 501 synthesis chemiosmotic mechanism, 69, 707 of cRNA, 65, 725 tetrapropylammonium bromide, interaction, 63, 298 vanadate ion contamination, 63, 10 Adenosine [a-32P]triphosphate, reaction mixture of polynucleotide kinase, 65, 481 of terminal transferase, 65, 508, 523, 524 Adenosine [y-32P]triphosphate preparation, 65, 67, 80, 242, 510, 515-519, 682; 69, 303 11 reaction mixture, of polynucleotide kinase, 65, 29-31, 67, 80, 242, 267,268, 508, 510, 511,521, 557, 584, 648, 669, 683 Adenosylhomocysteinase, coupled enzyme assay, 63, 36 S-Adenosylhomocysteine, as inhibitor, 77, 261, 272 S -Adenosyl-L-homocysteine assay, 63, 32 product in phosphatidylcholine synthesis, 71, 581 S-Adenosyl-L-homocysteine hydrolase, 71,134 S -Adenosylmethionine in S-adenosyl-L-methionine decarboxylase assay, 79, 347, 348 assay, 63, 32 ion-exchange paper chromatography, 67, 4 as substrate, 77, 257, 263, 267 tritium-labeled, 63, 36 S -Adenosyl-L-methionine alkaline hydrolysis, 71, 585 conversion to phosphatidylcholine, 71, 581 radioactive, 71, 134 transfer of methyl groups, 71, 582 S-Adenosylmethionine decarboxylase assay, 79, 347-349 induction, 79, 345 inhibition by interferon, 79, 343, 344 S -Adenosyl-L-methionine:phosphatidylethanolamine N-methyltransferases, see Phosphatidylethanolamine Nmethyltransferase Adenovirus, 68, 375 deoxyribonucleic acid binding protein, radioiodination, 73, 120 DNA isolation, 79, 313 termini labeling, 65, 41, 42 preparation, 79, 313 R loop mapping, 65, 761-763 transcription maps, 65, 750-768 early, 65, 761, 764-766 total, 65, 766-768 Adenovirus 2 cleavage sites, of restriction endonucleases, 65, 2-11 (4-Adenylyl)-l-butylcobalamin DNA cleavage products with restriction endonucleases, 65, 2-11, 773 with B a m H I , 65, 176-178 with EcoRI, 65, 176, 177, 317, 319, 769 with S m a I, 65, 769 with SstI, 65, 173 preparation, 65, 772, 773 growth and purification, 65, 771 storage buffer, 65, 771 transcription units by nascent chain analysis, 65, 775-778 Adenovirus 5 DNA, transforming activity, 65, 832, 833, 836 Adenovirus SA7 DNA, simian, tumor induction, 65, 838, 839 Adenylate cyclase, 64, 218; 79, 162, 163 activity, 79, 167 characterization of/3-adrenergic receptor antibodies, 74, 464471 assay, 74, 464; 79, 164-168 binding subunit, 79, 167 catalytic subunit, 79, 167 inhibition, 63, 395 purification, 74, 469 receptor, 79, 167 Adenylate kinase contaminant, in phosphotransferases, 63, 7 coupled assay, 63, 6, 32 enzyme contamination, 64, 24 inhibition, 63, 7, 398, 401,483 isotope exchange properties, 64, 8 mass action ratio, 63, 18 mechanism, 63, 18 mutant, 72, 694 photophosphorylation, 69, 656 quenching problem, 63, 16 substrate, metal-ion complex, 63, 259 Adenyl cyclase, see Adenylate cyclase Adenylosuccinate lyase mechanism, 63, 51 product inhibition, 63, 432 Adenylosuccinate synthetase coupled enzyme assay, 63, 32 mechanism, 63, 51, 157 (4-Adenylyl)-l-butylcobalamin, 63, 399 5'-Adenylylimidodiphosphonate 5'-Adenylylimidodiphosphonate inhibition, 63, 398 structure, 63, 403 5'-Adenylyl-fl,~/-methylenediphosphonic acid, 63, 481 Adipocyte from cultured 3T3 cells, hormonesensitive lipase, 71, 636 derived from 3T3-L1 preadipocytes, 72, 720 hamster, 72, 325 hormone-sensitive lipase, 71, 636 human, 72, 325 perifusion, 72, 707 accumulation of cyclic-AMP, 72, 713 apparatus, 72, 708 evaluation of technique, 72, 710 lipolytic response, 72, 712 plasma membranes isolation, 72, 747 amount of plasma membrane per adipocyte, 72, 751 chemical markers, 72, 751 cholesterol:phospholipid ratio, 72, 751 density gradient centrifugation, 72, 750 endoplasmic reticulum contamination, 72, 747 marker enzymes, 72, 751 Adipose tissue, see a l s o Adipocyte source of hormone-sensitive lipase, 71, 627, 636 Adjuvant bacterial, 76, 60 B o r d e t e l l a p e r t u s s i s vaccine, 76, 157 choice of injection route, 76, 112, 113 Freund, see Freund's adjuvant immune response, 70, 60, 107, 108 immunization, 70, 59, 60 intradermal immunization, 73, 47, 48 mechanism of action, 70, 107, 108 types, 70, 59, 60 use of polymer for macromolecules, 73, 57-75, see a l s o specific type Ad2* ND1 virus genomic structure, 65, 469 mRNAs, sandwich hybridization analysis, 65, 473 12 Adogen 464, 65, 332, 336 ADP, see Adenosine 5'-diphosphate Adrenal gland, iron-sulfur protein, 69, 230, 238 fl-Adrenergic receptor, see a l s o flAdrenergic receptor antibody antibody production, 74, 463 classes, immunogenic specificity, 74, 470-471 purification, 74, 461-463 affinity chromatography, 74, 462-463 crude extract preparation, 74, 460-461 particulate membrane fraction, 74, 461 solubilized membrane fraction, 74, 462, 468 tissue source, 74, 463 fl-Adrenergic receptor antibody, see a l s o fl-Adrenergic receptor antiserum fractionation, 74, 465-466 applications, 74, 471 immunogen preparation, 74, 460-463 production, 74, 463 radioimmunoassay adenylate cyclase activity studies, 74, 464-471 assay, 74, 464 procedure, 74, 464--465 results, 74, 464--465, 467-471 binding inhibition studies, 74, 464-467 label, choice, 74, 464 procedure, 74, 464 results, 74, 465-467 serum fractionation, 74, 465-466 specificity, 74, 467-471 Adrenocorticotropic hormone, see Adrenocorticotropin Adrenocorticotropin, 80, 365 immunogen preparation, 70, 97, 161, 162 Adriamycin immunogen preparation, 70, 96 radioimmunoassay, 73, 102, 275 radioiodination, 73, 102 Adsorption, nonspecific antiserum, 70, 122-124 13 Adsorption effect, lipolysis, 64, 345-353, 365, 375 Adsorption scanner, ultracentrifuge, improvements, 61, 99, 100 Affinity adsorbent, for leukocyte interferon, 78, 51 Affinity chromatography, 68, 439, see also specific substance aminohexyl-agarose, 77, 265 bacterial coupling factor, 69, 318 with S -p -bromobenzylglutathioneagarose, 77, 299 with butyl-agarose, 77, 349 Con A-Sepharose, 77, 245 3,4-dimethoxy-5-hydroxyphenylethylamine, as ligand, 77, 270 enzyme conjugate purification, 73, 168-171 glutathione as ligand, 77, 236 preparation, 77, 236 purification, 77, 236 human immune interferon, 78, 540-545 hydrophobic, mouse immune interferon, 78, 547-549 with phenyl-agarose, 77, 329 poly(dA) tailing procedure, 65, 492 purification, of human placental AP endonuclease, 65, 221 of pyrimidine dimer correndonucleases, 65, 187, 188 Affinity cytochemistry, avidin-biotin complex, 62, 308-315 Affinity electrophoresis, association constant measurement, 70, 30, 31 Affinity gel, in enzyme immunoassay, 73, 523-542, see also Steric hindrance enzyme immunoassay Affinity labeling active-site-directed, 72, 587 with o~-bromoacetamido fatty acids, 72, 587 CoA derivatives, 72, 580 Affinity method, see also specific substance using argininal derivatives, 80, 842-848 adsorbents handling, 80, 848 preparation, 80, 844, 845 Agarose properties, 80, 845, 846 quantitation of affinity, 80, 848 Affinity sorbent, for leukocyte interferon, 78, 51 Aflatoxin B, cytochrome P-450 activation, 74, 271 Aflatoxin B~, DNA damage and repair, 65, 247 AFP, see al-Fetoprotein African fish oocyte, see Tilapiamosaiabica peters oocyte African green monkey kidney cell, see Vero cell Agalactoorosomucoid, radioiodination, 70,222 Agammaglobulinemia, lymphoblast, immunoglobulin class released, 73, 592,593 Agar, washing, 69, 39 Agar electrophoresis, of hemoglobin S, 76, 787 Agaric acid effect on acetyl-CoA carboxylase, 72, 497 on ketogenesis, 72, 504 on lipogenesis, 72, 497,499, 501-503 in isolated hepatocytes, 72, 499, 503 in vivo, 72, 503 on redox state, 72, 505 in Fomes lacricis, 72, 497 Agarose choice for electroimmunoassay, 73, 35O commercial source, 65, 732 low melting temperature in DNA fractionation, 65, 358-363 restriction endonuclease digestion, 65, 362 N-Z bottom, 68, 434 PPO impregnation procedure, 65, 368-369 preparation of DNA-polyacrylamide, 65, 121 removal of unreacted [~-32P]ATP, 65, 268 structure, 73, 360-362 Agarose chromatography Agarose chromatography purification, ofDpn endonucleases, 65, 139 of Hpa II endonuclease, 65, 159, 160 of Hph I endonuclease, 65, 164 of SstI endonuclease, 65, 172 of venom phosphodiesterase, 65, 282 removal of gel contaminants from DNA framents, 65, 887 Agarose gel electrophoresis, 68, 15, 152, 178, 365, 422, 485; 79, 115-118 alkaline gels electrophoresis buffer, 65, 482, 732 loading buffer, 65, 732 procedure, 65, 733-734 analysis, of DNA restriction fragments, 65, 20, 30, 31, 104, 105, 118, 133, 134, 171, 177, 320-322, 469, 482, 484, 773 assay of restriction endonuclease, 65, 2O of Bst 1503, 65, 168 of DpnI and DpnII, 65, 142-143 ofHpa, 65, 153 sensitivity, 65, 27, 28 buffer, 65, 154, 194, 195 cloning hormone genes, 68, 88 cylindrical gel, determination of DNA digestion, 65, 772 DE-52 column, 68, 178 DNA, removal from gels, 65, 837--838 DNA nicking assay, 65, 194, 195 efficiency, of deoxyribonucleic acid recovery, 68, 178 glyoxalated nucleic acids, 65, 384, 385 horizontal apparatus, 65, 731 gel casting, 65, 733 materials and buffers, 65, 732 two-dimensional, 65, 734 limitations, in resolution, 68, 191 molecular weight determinations, of nucleic acids, 65, 388 neutral gels electrophoresis buffer, 65, 732 loading buffer, 65, 732 procedure, 65, 732, 733 14 plasmid DNA, 79, 610, 611 plasmid purification, 68, 265 RNA, 79, 428, 429 Southern blot technique, see Southern blot technique of 5' terminal labeled RNA, 65, 82-85 two-dimensional, 65, 358-363, 734, 747, 748 band detection and isolation, 65, 361, 362 gel pouring, 65, 359, 361 principles, 65, 358, 359 procedure, 65, 361 vertical tie-lines, 65, 747, 748 AG-1732 cell assay of interferon, 78, 473 cytopathic effect inhibition assay, 78, 389 Agglutination assay, for coated cells, 73, 337 reverse, for antigen assay, 73, 320 Agglutinator, mouse, 74, 129, 135-136 production, 74, 135 properties, 74, 135-136 Agglutinin, see also Lectin Ricinus communis, radioiodination, 73, 118 wheat germ, radioiodination, 73, 118 Agglutinin disease, cold, 70, 35--40 Aggregation, enzyme, 64, 387, 388 small-angle X-ray scattering studies, 61, 239, 240 AGLME, see Acetylglycine-lysine methyl ester Agmenellum quadruplicatum MNNG treatment, 69, 42 mutagenesis, 69, 41 mutant types, 69, 43-45 Agrobacterium tumefaciens, restriction endonucleases (Atu AI, Atu BI, Atu II, Atu CI), 65, 2 A549 human cell, virus yield-reduction assay for interferon, 78, 359, 366, 367 AimI restriction endonuclease, 65, 2 A23187 ionophore, chloroplast ion fluxes, 69, 599, 603 AIP method, comparative assays, 74, 72, 73, 75 Air pollution, carboxyhemoglobin, 76, 716 15 Alcohol dehydrogenase results, comparative, 74, 72, 73 standard curve, 74, 71-73 enzyme immunoassay, 73, 164 precipitating titer, measurement, 73, 317-319 196-208 immobilization, 70, 165 Z-Alanine 2-naphthy] ester, substrate immune reaction, assay, 74, 185, 186 for leukocyteelastase, 80, 583 immunogenicity, 70, 86, 87 D-Alanyl-p-hydroxy-D-phenylglycine inhibition, 63, 37 diketopiperazine, near-UV circular lipid interaction, 64, 354, 377,382 dichroism, 61, 362, 363 lung perfusion, 77, 113-115 n-Alanyl-L-leucyl-L-lysyl-7-amino-4-triflumethylated, immunogen preparation, oromethylcoumarin, assay of 70, 79, 82 plasmin, 80, 358 passive hemolysis inhibition L-Alanyl-L-phenyl-L-lysine-4-nitroanilide, immunoassay, 74, 164 hydrolysis perfusion, 77, 96, 100 by Factor Xa, 80, 351 in perfusion fluid, 77, 88 by plasmin, 80, 352 radioiodination, 70, 212, 226, 245-246 by thrombin, 80, 347 secreting cells, 73, 335-338 by trypsin, 80, 356 tolerance, induced, 70, 62 by urokinase, 80, 352 L-Alanyl-L-phenyl-L-lysyl-7-amino-4-met- Albumin-agarose binding of human interferons, 78, hylcoumarin, assay of coagulation 576--582 proteases, 80, 358 hydrophobic ligands, structure, 78, Alanylproline, 64, 225, 226 578 Alberty nomenclature, {}3,43 preparation, 78, 577-579 Albumin, 80, 73, see also Ovalbumin; with serum albumin, 78, 579 specific types Alcohol behavior, in gel diffusion, 70, 178, 179 equilibrium isotope effect, 64, 108-111 binding to fibroblast interferon, 78, immunogen preparation, 70, 97-100 453 long-chain, analysis by liquid bovine chromatography-mass antibody, enzyme immunoassay, spectrometry, 72, 77 73, 164 products of ozonide cleavage, 72, 258 immunogen preparation, 73, 64-71 sulfation serum, immune reaction kinetic aliphatic, assay, 77, 207 study, 74, 189-196 primary, 77, 206 as carrier in immunization, 70, 59 secondary, 77, 206 efficiency, 70, 82 Alcohol dehydrogenase, 77, 18 enzyme stabilizing agent, 63, 10 abortive complex, 63, 420 erythrocyte binding, calorimetry, 61, 265 affinity chromatography, of NAD, 66, 43-48 fluorescence immunoassay, 74, 32, 48 assay, of NAD, 66, 41, 42, 80 functional groups, for conjugation, 70, 87, 88 binding, to agarose-bound nucleotides, 66, 196-208 human coupled assay, 63, 34 assay, by indirect quenching fluoroimmunoassay, 74, 69-73 cryoenzymology, 63, 338 in biological fluids, 74, 73 equilibrium perturbation, 64, 120, 123 dilution curves, 74, 69-70 AKR virus, radioiodination, 70, 212 ~-Alanine, precursor of pantothenate synthetase, 62, 215, 218, 219 Alanine dehydrogenase, binding, to agarose-bound nucleotides, 66, Alcohol dehydrogenase half-site reactivity, 64, 184 inhibitor, 63, 398, 408 isotope effect, 63, 110 isotope exchange, 64, 8, 28, 29 mechanism, 63, 51 product inhibition, 63, 436 reaction intermediates, stopped-flow absorbance studies, 61, 319 substrate effect, 63, 494 substrate inhibition, 63, 507 Alcohol ester gas-hquid chromatography, 72, 264 reductive ozonolysis, 72, 258, 264 Alcoholism, superoxide dismutase levels, 74, 360, 367-369 Aldehyde assay, 63, 34 autofluorescence, 74, 91 dimethylacetals, 72, 268 long-chain, bioluminescence assay, 72, 338 products of ozonide cleavage, 72, 258, 259, 260 Aldehyde dehydrogenase, 77, 18, see also Coenzyme A-linked aldehyde dehydrogenase inhibition, slow-binding, 63, 450, 465 from liver, 71,772 affinity chromatography, 71, 775 assay, 71, 778 isozymes, 71, 772 mitochondrial and cytoplasmic forms, 71, 773 properties of horse, sheep, and human liver isozymes, 71, 773, 774 purification, 71, 773 sulfhydryl group sensitivity, 71, 773 susceptibility to disulfiram, 71, 773 Aldehyde ester gas-liquid chromatography, 72, 264 from reductive ozonolysis, 72, 258, 259, 268 Aldehyde hydrate, equilibrium isotope effect, 64, 108-110 Aldehyde:NAD ÷ oxidoreductase, see Aldehyde dehydrogenase Aldehyde reductase assay, 71, 269 16 from B r a s s i c a oleracea, 71, 269 specificity, 71,272 Aldolase, see also Fructose-bisphosphate aldolase anomeric specificity, 63, 374 direct kinetic determination, 63, 378 coupled assay, use, 63, 34 radioiodination, 70, 212 Aldomet, see aMethyldihydroxyphenylalanine Aldose-l-epimerase, assay, 63, 33 Aldosterone, 63, 401,404 immunogen preparation, 70, 101 radioimmunoassay, 73, 108 receptor, 63, 401 release, 80, 427, 428 as substrate, 77, 213 Aldosterone 18,21-diacetate, immunogen preparation, 70, 101 Aldrin, radioimmunoassay, 73, 108 Alfalfa, ferredoxin, 69, 232 amino acid sequence, 69, 237, 238 Algae, see also specific types isolation of ferredoxin assays, 69, 232, 233 extraction, 69, 233 purification, 69, 233, 234 photorespiration assay, 69, 460, 461 plastoquinones, 69, 377, 380 Alkali denaturation test, for abnormal hemoglobins, 76, 747 Alkaline phosphatase, 68, 76, 258, 325; 71, 242, 745 activity assay, 70, 433 analysis, of NAADP, 66, 110-112 assay, 71, 733 of (2'-5')-oligoadenylate synthetase, 79, 151, 157, 158 of 2'-phosphodiesterase, 79, 161 bacterial assay, 65, 681 commercial source, 65, 681 dephosphorylation, of nucleic acid, 65, 237, 241, 242, 481, 584, 653,683, 695 elution, of DNA from gels, 65, 44, 51 of H p h I endonuclease, 65, 164 substrate specificity, 65, 190 17 tritium derivative method, 65, 640, 641, 645, 647,648, 653, 658, 660 calf intestine, 68, 86 catalytic properties, 65, 79 digestion procedure, 65, 79, 80, 665 characterization, of 2'P-ADP, 66, 117, 118 conjugate preparation, for immunoassays, 70, 432, 433 conjugate stability, 73, 162, 163 conjugation to antibody, 73, 150-152, 165, 166, 409, 495-497, 659, 660 applications, 73, 282-288 coupled assay, 63, 34, 36 cryoenzymology, 63, 338 dephosphorylation of DNA, 65, 505, 520, 521 enzyme-linked immunosorbent assay, 74, 591, 592 flip-flop mechanism, 64, 185 immunoassay, 73, 384-387 inactivation procedure, 65, 267 inhibitors, 65, 75-77 kinetic parameters, 63, 238 negative cooperativity, 64, 185 pH effects, 63, 215 phosphate-water exchange, 64, 83 phosphatidic acid, 69, 301 phosphorus-31 magnetic resonance, 64, 83 radioiodination, 73, 121 precursor form, 73, 120 reaction pathway, 64, 76 release by phospholipase C, 71,732 spectrokinetic probe, 61,323 Alkane-a,w-dioic acids, 71,411 Alkane-l,2-diol reduction of a-hydroxy acids, 71, 272 Alkylation, half-site reactivity, 64, 183 Alkyl isocyanide binding to chains, 76, 633, 634 to heme, 76, 470 to hemoproteins, 76, 424 Alkylisocyanide hemoglobin, preparation, 76, 62-63 Alkyl ketone, as activator, 77, 177 N-A|kylnicotinamide, 63, 396, 398 Alumina Alkylphenyl-silica, high-performance liquid chromatography, 79, 14 Alkylphosphonic acid, phosphorus determination, 72, 292 Allergen immobilization, 73, 208, 654 procedure, 70, 380 specific antibody, see Antibody, blocking Allergy, screening test, 70, 376-387 Alloantiserum, enzyme immunoassay, 73,520 d -Allobisnorbiotin characterization, 62, 387-390 isolation, 62, 385-387 Allopurinol, slow-binding inhibition, 63, 463, 464 Allosterism, see a l s o Site-site interaction cascade control systems, 64, 298-322 cooperativity, 64, 139, 161, 162 isotope exchange probes, 64, 24 isotope trapping method, 64, 58 phosphotransferases, 63, 293 PRPP:ATP phosphoribosyltransferase, 63, 9 substrate inhibition, 63, 43,500, 501 Alloxanthine, slow-binding inhibition, 63, 463, 464 ALME, see N ~-Acetyl-L-lysine methyl ester Alprenolol-Sepharose column, purification of fl-adrenergic receptors, 74, 463 Alternative product inhibition, 63, 436 Alternative substrate, 63, 10, 145, 146, 236, 372-374, 486-500; 64, 325-340, 342, 343 graphical method, 63, 490-499 common-product, 63, 494--498 constant-ratio, 63, 498-499 one-product, 63, 491-494 numerical method, 63, 487-490 A l u I restriction endonuclease, 65, 21, 27, 363, 394, 481, 570, 573, 799 recognition sequence, 65, 3, 555, 572 Alumina assay, of (2'-5')-oligoadenylate synthetase, 79, 152-154, 157 of cyclic adenosine monophosphate, 79, 164-165 Alumina recovery of (2'-5')-oligoadenylate, 79, 153 separation of (2'-5')-oligoadenylate, 79, 152-154 Aluminum chloride, preparation, ofpchlorophenyl phosphodichloridate, 65, 615 A m a r a n t h u s , triazine-resistant mutants, 69, 702 Amberlite MB-1, deionizing acrylamide, 65, 310 Amber suppressor tRNA, preparation, 65, 804, 805 t-AMCHA, see t r a n s - 4 Aminomethylcyclohexane-1-carboxylic acid Amebiasis, invasive, antibody, enzyme immunoassay, 73, 164 Amidase, 77, 333-344, see also Carboxylesterase assay, 77, 405409 with acetanilide, 77, 406-409 with aniline derivatives, 77, 405-407 with butanilicaine, 77, 407 diazotization, 77, 405-407 fluorometric, 77, 408, 409 with 4-nitroacetanilide, 77, 407 with paracetamol, 77, 408, 409 with phenacetin, 77, 408, 409 with p-phenetidine, 77, 405-407 spectrophotometric, 77, 407 properties, 77, 342-344 purification, 77, 337-342 solubilization, 77, 337 Amide, hydrolysis, isotope effect, 64, 95, 103 Amido black gel staining, 69, 369 as hemoglobin stain, 76, 18 Amikacin fluorescence immunoassay, 74, 79 radioimmunoassay, 73, 101 radioiodination, 70, 234 Amine equilibrium isotope effect, 64, 108, 110 fluorescent amine, measurement of membrane hpH, 69, 542,543 calculations, 69, 544 materials, 69, 542, 543 18 procedure, 69, 543 validity of method, 69, 544, 545 low molecular weight, as interferon inducers, 78, 293-295 stimulation of proton uptake, 69, 545 calculation, 69, 546 materials and methods, 69, 545, 546 validity and drawbacks of method, 69, 546 treatment of plastic tubes, 73, 234, 235 Amine oxidase, in intestinal epithelium, 77, 156 Aminex A-5, synthesis, of oligodeoxyribonucleotides, defined sequence, 65, 695 Aminex A-27, nucleotide purification, 64, 128, 134 Amino acid, see also specific types analysis automated, 79, 20-25 buffers, 79, 24, 25 fluorescamine, 79, 20-25, 25-27 fluorescence, 79, 20-25 gel protein bands, 79, 25-27 human fibroblast interferon, 79, 26 instrumentation, 79, 21-23 leukocyte interferon, 79, 23-25 peptides, 79, 20-25 picomole level, 79, 20-25 protein, 79, 20-25 protein bands, 79, 25-27 reagents, 79, 21-25 aromatic, side-chain structures, 61, 473 assay, 63, 33 cell-free protein synthesis, 65, 806 composition of bacteriochlorophyll a protein, 69, 339, 340 of Fe protein, 69, 774, 775 of flavodoxins, 69, 399, 401, 402 of Mo-Fe protein subunits, 69, 766-768 leukocyte interferon, 78, 463, 471, 480 lymphoblastoid interferon, 78, 463 equilibrium isotope effect, 64, 108 19 gut perfusion, 77, 127 mixture, for globin-synthesis studies, 76, 758 radioactive, cellular protein labeling, 79, 327-329 residues acetylation, 63, 218 analogs, 63, 397, 399, 400 dielectric constant effects, 63, 211, 212 direct titration, 63, 212-214 heats of ionization, 63, 209-211 identification, 63, 209-220 pK values, 63, 209, 210 sequence determination automated, 79, 31--48 manual, 79, 27-31 subnanomolar level, 79, 31--48 ferredoxins, 69, 235-238 human fibroblast interferon, 78, xxi; 79, xxxi human leukocyte interferon, 78, xx, 471; 79, xxxii, xxxiii leghemoglobins, 69, 819 sequencing, computer data analysis, 65, 598-609 D-Amino-acid oxidase assay, 63, 34 cryoenzymology, 63, 338 L-Amino-acid oxidase, assay, 63, 34 Amino acid polymer, as immunogens, 70, 50, 51 Aminoacridine, covalently bound, 69, 518 9-Aminoacridine fluorescence emission spectra, 61, 403 as fluorescence probe, 69, 516 measurement of membrane hpH, 69, 542-544, 635 Aminoacyl-transfer ribonucleic acid, binding, at recognition site, 61, 250 4-Amino-5-aminomethyl-2-methylpyrimidine, synthesis, 62, 75 2-Aminobenzimidazole, fluorescence immunoassay, 74, 11 p-Aminobenzoic acid identification, by Sephadex chromatography, 68, 503 7-Aminoheptanoic acid synthesis of aminopterin, with 13Clabeled in benzoyl carbon, 66, 536 thin-layer chromatography, 66, 442 2-Aminobenzoic acid, glucuronidation, 77, 176 2-Amino-4-benzoic acid, inhibitor, of dihydropteroate synthetase, 66, 574 3-Amino-4-benzoic acid, inhibitor, of dihydropteroate synthetase, 66, 574 4-Aminobenzoic acid, 77, 274 p-Aminobenzoylglutamic acid high-performance liquid chromatography, 68, 455 Sephadex chromatography, 66, 503 thin-layer chromatography, 66, 442 (Aminobenzyloxymethyl)cellulose, 68, 208-210, 446 4-Aminobutyric acid, effect on conformation of Glu-plasminogen, 80, 377, 378 ~-Aminocaproic acid, 80, 40 analogs, effects on human plasminogen, 80, 376-378 effect on Glu-plasminogen, 80, 374, 377 on plasmin kinetics, 80, 382 Aminocellulose, immunoadsorption, 70, 338, 339 a-Amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid, as inhibitor, 77, 249 9-Amino-6-chloro-2-methoxyacridine, energized state, 69, 516, 517 4-Amino-5-cyano-2-methylpyrimidine, synthesis, 62, 75 4-Aminodeoxy-7,8-dihydro-10-thiopteroic acid, inactivity, inhibitor of dihydrofolate synthetase, 66, 580 8-Amino-7,10-dimethylisoalloxazine, affinity chromatography, 66, 339 3-Aminoenolpyruvate-2-phosphate, 63, 397, 399 2-Aminofluorene, as substrate, 77, 279 fl-Aminoglutaryl-L-aminobutyrate, 63, 397, 399 Aminoglycoside, radioimmunoassay, 73, 101 7-Aminoheptanoic acid, effect on conformation of Glu-plasminogen, 80, 378 6-Aminohexanoic acid 6-Aminohexanoic acid effect on a2-antiplasmin assay, 86, 398 on conformation of Gluplasminogen, 80, 377, 378 on plasmin-a2-antiplasmin reaction, 80, 405 Aminohexyl-Sepharose, preparation, 77, 265 2-Amino-4-hydroxy-6-hydroxymethylpteridine pyrophosphate biological evaluation, 66, 555 inhibition, 66, 555, 556 synthesis, 66, 554, 555 8-Aminolevulinate synthetase, inhibitor, 63, 400 ~-Aminolevulinic acid, ion-exchange paper chromatography, 67, 4 Aminomalonate, 63, 400 trans -4-Aminomethylcyclohexane-l-carboxylic acid effect on Glu-plasminogen, 80, 374-378 on plasmin kinetics, 80, 382 2-Amino-2-methyl-1,3-propanediol, as chromatography buffer, 76, 32-33 (4-Amino-2-methyl-5-pyrimidinylmethyl)5-(2-hydroxyethyl-4-methyl)thiazolethione synthesis, 62, 75, 76 8-Aminooctanoic acid, effect on conformation of Glu-plasminogen, 80, 377, 378 5-Aminopentanoic acid, effect on conformation of Glu-plasminogen, 80, 378 Aminopentyl-Sepharose, purification, of B a m HI, 65, 150 Aminopeptidase, cytosol, small-angle X-ray scattering, 61, 226 2-Aminophenol glucuronidation, 77, 176 as substrate, 77, 384, 385 4-Aminophenol, electron transport, 69, 433 Aminophenyl derivative, use, in immunogen preparation, 70, 98, 154, 155 5 '-(4-Aminophenylphosphoryl)uridine 2' (3')-phosphate-agarose, purification, of deoxyribonuclease, 65, 719 20 3-Aminophthalhydrazide, see Luminol 1-Amino-D-proline, natural antagonist of vitamin Be, 62, 487-489 Aminopterin, 63, 396, 399 characterization, 66, 537, 538 inhibition, tight-binding, 63, 462 synthesis, 13C-labeled in benzoyl carbon, 66, 536, 537 3-Aminopyridine adenine dinucleotide preparation, 66, 82, 83 properties, 66, 85-87 3-Aminopyridine adenine dinucleotide phosphate preparation, 66, 83-85 properties, 66, 85-87 Aminopyrine, demethylation, carbon dioxide formation, 77, 4, 5 7-Amino-10-(l'-D-ribityl)isoalloxazine, affinity chromatography, 66, 339 0-Amino-D-serine, natural antagonist of vitamin B6, 62, 492-495 Aminotriazole, peroxidatic reaction, 77, 20 A m m i visnaga, culture, protoplasts, 69, 75 Ammonia amino acid analysis, 79, 27 aqueous, elution, of tritiated nucleoside trialcohols, 65, 644 assay, 63, 34 equilibrium isotope effect, 64, 108 Ammonia metmyoglobin, polarized absorption spectroscopy of, 76, 189 Ammonium acetate assay, of mung bean nuclease, 65, 264, 266 chemical cleavage, of DNA, 65, 538 DNA precipitation, 65, 557, 565 preparation, of [5'-32P]nucleoside 3',5'-diphosphates, 65, 67 purification, of mung bean nuclease, 65, 269, 270 reaction mixture, for cell-free protein synthesis, 65, 806 removal, of DNA, from gels, 65, 304, 373, 506, 515, 526, 565 stop solution, of polynucleotide kinase, 65, 522, 683 of restriction endonuclease, 65, 724 of S 1 nuclease, 65, 729 21 of terminal transferase, 65, 523 synthesis, of oligodeoxyribonucleotides, defined sequence, 65, 694 Ammonium chloride M-9 medium, 65, 798 preparation, of cell-free protein synthesis system, 65, 805 uncoupling, by gramicidin, 69, 720 Ammonium formate analysis, of oligonucleotide 3'dialcohols, 65, 658, 659 of 32p-labeled RNA fragments, 65, 673-676 synthesis of oligodeoxyribonucleotides, defined sequence, 65, 695 Ammonium hydroxide, preparation, of deoxyoligonucleotides, 65, 619 Ammonium ion assimilation, by K.

P n e u m o n i a e , 69, 50, 51 transport, 69, 720-721 Ammonium pentachlorooxomolybdate reagent, 72, 189 Ammonium persulfate, 65, 121, 241, 302, 303, 309, 452, 540-543, 573, 671, 685, 782, 800, 809 cytochrome assay, 69, 182 Ammonium 1-pyrrolidine dithiocarbamate, Mn extraction, 69, 362 Ammonium sulfate analysis, of 32p-labeled RNA fragments, 65, 673, 674, 676 fractionation, preparation of immunoglobulin G, 74, 26, 67, 267, 285, 413,440 high molecular weight hemoglobin precipitation by, 76, 51-53 inhibition, 63, 37 purification of CAP, 65, 859, 862 of E.

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