By Bernard N. Fields and Rudolf Jaenisch (Eds.)
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SPECTOR FIGURE 2. Eco RI cleavage of HCMV DNA. 8% agarose gel. The numbers assigned to the bands of the restriction pattern are shown to the left of the gel. 3. MOLECULAR CLONING OF CYTOMEGALOVIRUS GENOME 27 were then cleaved with Eco RI, and the fragments were separated by electrophoresis through agarose gels to determine the size and identity of the inserted sequences. Assignment of a fragment to a specific HCMV band was made on the basis of comigration of the cloned fragments with bands from an Eco RI digest of HCMV DNA (Figure 2 ) .
And Szybalski, W. ), Cold Spring Harbor 20 DAVID BOTSTEIN Laboratory, pp. 45-82. 9. Hilliker, S. D. thesis, Mass. Institute of Technology. 10. , and Schaefer, F. ), Cold Spring Harbor Laboratory, pp. 469-475. 11. , and Hendrix, R. (1974) J_. Mol. Biol. 90:20-25. 12. A. Jr. (1975) J. Mol. Biol. 9L· 439-462. 13. M. (1975) J. Mol. Biol. 9L· 421-438. 14. M. S. and Fox, C F . A. Benjamin, pp. 363-384. 15. M. , and Botstein, D. (1975) J_. Mol. Biol. 98_: 413-424. 16. , Langenaur, C , and Agabian, N.
The bands of the Eco RI digest also showed variations in intensity, suggesting that some viral sequences may co-migrate on the gel (see Figure 2 ) . In accordance with 28 JOYCE C. TAMASHIRO AND DEBORAH H. SPECTOR FIGURE 3. A-D. Analysis of a representative collection of the cloned fragments obtained. Recombinant plasmid DNA was purified, cleaved with Eco RI, fractionated on agarose gels, and stained with ethidium bromide. The cloned inserts correspond to HCMV Eco RI restriction bands. Panel A. Lane 1 contains an insert co-migrating with band la; 2, band lb; 3, band 2; 4, band 3; 5, band 6; 6, band 7; 7, band 8a.